Microscopy Facility | Department of Biology

Microscopy Facility

The Microscopy lab provides access to microscopy equipment and image processing resources. The lab’s core mission is enhance research capabilities and provide an environment that promotes undergraduate and graduate students research

For access to department calendars including conference rooms, microscopes, autoclaves, and greenhouses, e-mail bio@uncg.edu

Lab Equipment

All-in-One Fluorescence Microscope – Keyence BZ-X710

  • Available objectives:
    Position 1: PlanApo 2x/0.10 Infinity/WD 8.5
    Position 2: PlanApo 10x/0.45 Infinity/WD 4
    Position 3: PlanApo 20x/0.75 Infinity/WD 1
    Position 4: S PlanFluor ELWD 40x/0.60 Ph2
  • Available filters:
    Channel 1: GFP
    Channel 2: TxRed
    Channel 3: DAPI
    Channel 4: Brightfield/Phase contrast
    (Available upon request: Cy5)

Confocal/Widefield Microscope – Nikon AXR

Nikon AXR inverted frame dual point-scanning confocal/widefield microscope with AXR 2k Resonant Confocal, 4CH Hybrid GaAsP and PMT Detection, NIS-C, and an sCMOS camera visualized on an HP Z4 Enhanced Workstation with 31.5″monitor.

Objectives

  1. 10X, NA 0.45, air CFI60 Plan Apo Lambda D, W.D. 4.0mm, FOV 25mm, DIC
  2. 20X, NA 0.80, air, CFI60 Plan Apo Lambda D, W.D. 0.80mm, FOV 25mm, DIC, spring loaded
  3. 40x, NA 0.95, air, CFI60 Plan Apo Lambda D, W.D. (with correction collar) 0.17- 0.25mm, FOV 25mm, DIC, spring loaded
  4. 100x, NA 1.45, oil, CFI60 Plan Apo Lambda D

Excitation

  • 4 Line 405/488/561/640 Laser Unit for point-scanner
  • D-LEDI for eyepiece and camera

Detection

Incubation

  • Heat and CO2 available

Analysis Software

  • NIS-Elements software on supplied PC
  • Fiji

Booking Lab

To schedule training and time in the lab, please contact Lee Griffin by calling 336.334.4976, or email.

Lab Fees

User Hourly Rate Includes
Confocal $25.00 – $50.00 Training and Supervision
Keyence $10.00 – $20.00 Training and Supervision

Location

Sullivan Building – Room 353

 

Facility contacts
John Tomkiel Dean
Zhenquan Jia
Nick Ader

 

Questions
Lee Griffin
(336) 334-4976