The Microscopy Core Facility, housed in the Department of Biology, provides access to microscopy acquisition and analysis resources for UNCG researchers and classes.
For access to department calendars including conference rooms, microscopes, autoclaves, and greenhouses, e-mail bio@uncg.edu
Microscopes
All-in-One Fluorescence Microscope – Keyence BZ-X710
- Available objectives:
Position 1: PlanApo 2x/0.10 Infinity/WD 8.5
Position 2: PlanApo 10x/0.45 Infinity/WD 4
Position 3: PlanApo 20x/0.75 Infinity/WD 1
Position 4: S PlanFluor ELWD 40x/0.60 Ph2 - Available filters:
Channel 1: GFP
Channel 2: TxRed
Channel 3: DAPI
Channel 4: Brightfield/Phase contrast
(Available upon request: Cy5)
Confocal/Widefield Microscope – Nikon AXR
Nikon AXR inverted frame dual point-scanning confocal/widefield microscope with AXR 2k Resonant Confocal, 4CH Hybrid GaAsP and PMT Detection, NIS-C, and an sCMOS camera visualized on an HP Z4 Enhanced Workstation with 31.5″monitor.
Discover full information on FPbase.
Objectives
- 10X, NA 0.45, air CFI60 Plan Apo Lambda D, W.D. 4.0mm, FOV 25mm, DIC
- 20X, NA 0.80, air, CFI60 Plan Apo Lambda D, W.D. 0.80mm, FOV 25mm, DIC, spring loaded
- 40x, NA 0.95, air, CFI60 Plan Apo Lambda D, W.D. (with correction collar) 0.17- 0.25mm, FOV 25mm, DIC, spring loaded
- 100x, NA 1.45, oil, CFI60 Plan Apo Lambda D
Excitation
- 4 Line 405/488/561/640 Laser Unit for point-scanner
- D-LEDI for eyepiece and camera
Detection
- 4CH Hybrid GaAsP and PMT Detection for point-scanner
- ORCA Fusion BT sCMOS
Incubation
- Heat and CO2 available
Analysis Software
- NIS-Elements software on supplied PC
- Fiji
Booking Lab
To schedule training and time in the lab, email Nick Ader.
Lab Fees
| User | Hourly Rate | Includes |
|---|---|---|
| Confocal | $25.00 – $50.00 | Training and Supervision |
| Keyence | $10.00 – $20.00 | Training and Supervision |
Location
Sullivan Building – Room 353
Questions or Trainings
Nick Ader
Director, Biology Microscopy Facility