Publications | Rueppell Research Group



Amiri E., Waiker P., Rueppell O., Manda P. (2020) Using manual and computer-based text-mining to uncover research trends for Apis mellifera. Veterinary Sciences, 7:61. Link

Wagoner K.M., Millar J., Schal C., Rueppell O.  Cuticular pheromones stimulate hygienic behavior in the honey bee (Apis mellifera). Scientific Reports, 10: 7132. Link

Amiri E., Strand M.K., Tarpy D.R., Rueppell O. Honey bee queens and virus infections. Viruses, 12(3): 322. Link

Amiri E., Le K., Vega-Melendez C., Strand M.K., Tarpy D.R., Rueppell O. Egg-size plasticity in Apis mellifera: honey bee queens alter egg size in response to both genetic and environmental factors. Journal of Evolutionary Biology,33(4): 534-543. Link

Oppenheim S., Cao X., Rueppell O., Chantawannakul P., Krongdang S., Phokasem P., DeSalle R., Goodwin S., Xing J., Rosenfeld J. Whole Genome Sequencing and Assembly of the Asian Honey Bee Apis dorsata. Genome Biology and Evolution, 12(1): 3677-3683. Link

Wagoner K.M., Rueppell O.  U.S Patent No. 10,524,455 B2: Methods and Compositions for Inducing Hygienic Behavior in Honey bees.

DeLory T., Funderburk K., Miller K., Zuluaga-Smith W., McPherson S., Pirk C.W., Costa C., Weinstein-Teixeira E., Dahle B., Rueppell O. Local variation in recombination rates of the honey bee (Apis mellifera) genome among samples from six disparate populations. Insectes Sociaux, 67(1):  127-138. Link


Wagoner K.M., Rueppell O. U.S Patent No. 10,512,251 B2: Methods and Compositions for Inducing Hygienic Behavior in Honey bees.

Wagoner K.M., Spivak M., Hefetz A., Reams T., Rueppell O. Varroa mites and Deformed Wing Virus elicit hygienic behavior in honey bees through stock-specific changes in brood cuticular hydrocarbons. Scientific Reports, 9: 8753. Link

Amiri E., Seddon G., Zuluaga-Smith W., Strand M.K., Tarpy D.R., Rueppell O. Israeli Acute Bee Paralysis Virus: queen-worker interaction and potential virus transmission pathways. Insects, 10(1): 9. Link

Wu F., Ma C., Han B., Meng L., Hu H., Fang Y., Feng M., Zhang X., Rueppell O., Li J., (2019) Behavioral, physiological, and molecular changes in alloparental care givers may be responsible for selection response for female reproductive investment in honey bees. Molecular Ecology, 28: 4212-4227. Link

Waiker P., Baral S., Kennedy A., Bhatia S., Rueppell A., Le K., Amiri E., Tsuruda J., Rueppell O. Foraging and homing behavior of honey bees (Apis mellifera) during a total solar eclipse. The Science of Nature, 106:4. Link

Rueppell O., Kennedy A. Aging and Behavior in Honey Bees. In: “Encyclopedia of Animal Behavior, Second Edition”, J. Choe (ed.), Academic Press, ISBN13: 978-0128132517.


Amiri E., Kryger P., Meixner M.D., Strand M.K., Tarpy D.R., & Rueppell O. Quantitative patterns of vertical transmission of deformed wing virus in honey bees. PLoS One, 13(3): e0195283. Link 

Wagoner K.M., Spivak M., Rueppell O. Brood affects hygienic behavior in the honey bee (Hymenoptera: Apidae). Journal of Economic Entomology, 111(6): 2520-2530. Link

Lawhorn C.M., Shomaker R., Rowell J.T., & Rueppell O. Simple comparisons of differentially expressed gene lists may overestimate gene overlap. Journal of Computational Biology, 25(6). 606-612. Link

Quigley T.P., Amdam G.V., Rueppell O. Honey bee workers as models of aging. In: “Conn’s Handbook of Models for Human Aging”, J. Ram (ed.), Elsevier Press, Atlanta, p.533-547. Link

Langberg K., Phillips M., Rueppell O. Testing the effect of paraquat exposure on genomic recombination rates in queens of the Western Honey Bee, Apis mellifera. Genetica, 146(2), 171-178. Link


Amiri E., Strand M.K., Rueppell O. & Tarpy D.R. Queen quality and the impact of honey bee diseases on queen health: potential for interactions between two major threats to colony health. Insects, 8(2), 48. Link

Rueppell O., Yousefi B., Collazo J. & Smith D. Early life stress affects mortality rate more than social behavior, gene expression or oxidative damage in honey bee workers. Experimental Gerontology, 90: 19-25. Link

Wagoner K.M. & Rueppell O. Effects of steel foundation wire on elemental content and hygienic removal of honey bee (Apis mellifera) brood. Journal of Apicultural Research, 56(3):270-277. Link


Rueppell O., Kuster R. , Miller K. , Fouks B., Rubio Correa S., Collazo J., Phaincharoen M., Tingek S., & Koeniger N. A new metazoan recombination rate record and consistently high recombination rates in the honey bee genus Apis accompanied by frequent inversions but not translocations. Genome Biology and Evolution, 8(12): 3653-3660. Link

Simone-Finstrom M., Li-Byarlay H., Huang M.H., Strand M.K., Rueppell O., & Tarpy D.R. Migratory management and environmental conditions affect lifespan and oxidative stress in honey bees. Scientific Reports, 6: 32023. Link

Li-Byarlay H., Huang M.H., Simone-Finstrom M., Strand M.K., Tarpy D.R., & Rueppell O. Honey bee (Apis mellifera) drones survive oxidative stress due to increased tolerance instead of avoidance or repair of oxidative damage. Experimental Gerontology, 83(10): 15-21. Link

Rueppell O., Aumer D. & Moritz R.F.A. Ties between aging plasticity and reproductive physiology in honey bees (Apis mellifera) reveal a positive relation between fecundity and longevity as consequence of advanced social evolution. Current Opinion in Insect Science, 16: 64-68. Link


Thompson E., Everett J., Rowell J.T., Rychtar J. & Rueppell O. The evolution of cooperation is affected by the persistence of fitness effects, the neighborhood size and their interaction. Letters in Biomathematics, 2(1): 67-78. Link

von Wyschetski K., Rueppell O., Oettler J. & Heinze J. Transcriptomic signatures mirror the lack of the fecundity / longevity trade-off in ant queens. Molecular Biology and Evolution, 32(12): 3173-3185. Link

Rueppell O., Koenigseder F., Heinze J. & Schrempf A. Intrinsic survival advantage of social insect queens depends on reproductive activation. Journal of Evolutionary Biology, 28(12): 2349-2354. Link

Sadd B.M., Barribeau S.M., et al. (List of 142 Authors including Rueppell, O.) The genomes of two key bumblebee species with primitive eusocial organization. Genome Biology, 16:76. Link

Ross C., DeFelice D., Hunt G., Ihle K., Amdam G.V. & Rueppell O. Genomic correlates of recombination rate and its variability across eight recombination maps in the Western Honey Bee (Apis mellifera L.). BMC Genomics, 16:107. Link

Ihle K.E., Rueppell O., Huang Z.Y., Wang Y., Fondrk M.K., Page R.E., Amdam G.V. Genetic architecture of a hormonal response to gene knockdown in honey bees. Journal of Heredity,106: 155-165. Link

Michaud S., Boncristiani H.F., Gouw J.W., Strand M., Pettis J., Rueppell O., Foster L.J. Response of the honey bee (Apis mellifera L.) proteome to Israeli acute paralysis virus infection. Canadian Journal of Zoology, 93(9): 711-720. Link

Ross C., Rychtar J., Rueppell O. A structured population model suggests that long life and post-reproductive lifespan promote the evolution of cooperation. Journal of Theoretical Biology, 369:85-94. Link

Ross C., DeFelice D., Hunt G., Ihle K., Rueppell O. A comparison of multiple genome-wide recombination maps in Apis mellifera. Springer Proceedings in Mathematics & Statistics 109: 91-98. Link

DeFelice D.S., Ross C., Simone-Finstrom M., Warrit N., Smith D.R., Burgett M., Sukumalanand P., Rueppell O. Geographic variation in polyandry of the Eastern Honey Bee, Apis cerana, in Thailand. Insectes Sociaux, 62(1): 37-42, Link


Heinze J., Rueppell O. The frequency of multi-queen colonies increases with altitude in a Nearctic ant. Ecological Entomology, 39: 527–529. Link

Forkpah C., Dixon L.R., Fahrbach S.E., Rueppell O. Xenobiotic effects on intestinal stem cell proliferation in adult honey bee (Apis mellifera L) workers. PLoS ONE, 9(3): e91180. Link

Kuster R.D., Boncristiani H.F., Rueppell O. Immunogene and viral transcript dynamics during parasitic Varroa destructor (Anderson) mite infection of developing honey bee (Apis mellifera L) pupae. Journal of Experimental Biology, 217: 1710-1718. Link

Elsik C.G., Worley K.C., Bennett A.K., Beye M., Camara F.C., Childers C.P., de Graaf D.C., Debsyer G., Deng J., Devreese B., Elhaik E., Evans J.D., Foster L.J., Graur D., Guigo R., Hoff K.J., Holder M.E., Hudson M.E., Hunt G.J., Jiang H., Joshi V., Khetani R.S., Kosarev P., Kovar C.L., Ma J., Maleszka R., Moritz R.F.A., Munoz-Torres M.C., Murphy T.D., Muzny D.M., Newsham I.F., Reese J.T., Robertson H.M., Robinson G.E., Rueppell O., Solovyev V., Stanke M., Stolle E., Tsuruda J.M., Van Vaerenbergh M., Waterhouse R.M., Weaver D.B., Whitfield C.W., Wu Y., Zdobnov E.M., Zhang L., Zhu D., and Gibbs R.A. Finding the missing honey bee genes: lessons learned from a genome upgrade. BMC Genomics, 15(1):86. Link

Rueppell O. The architecture of the pollen hoarding syndrome in honey bees: Implications for understanding social evolution, behavioral syndromes, and selective breeding. Apidologie, 45: 364-374.  Link

Dixon L.R., Kuster R.D., Rueppell O. Reproduction, social behavior, and aging trajectories in honey bee workers. AGE, 36: 89-101. Link


Wagoner K.M., Boncristiani H.F., Rueppell O. Multifaceted responses to two major parasites in the honey bee (Apis mellifera). BMC Ecology, 13:26. Link.

Ross C. R., Rueppell O., Rychtar J. A spatially organized population model to study the evolution of cooperation in species with discrete life-history stages. Springer Proceedings in Mathematics & Statistics, 64: 147-154. Link.

Crowe M.L., Rychtar J., Rueppell O., Chhetri M., Remington D.L., Gupta S.N. Proving the “proof”: Interdisciplinary undergraduate research positively impacts students. Springer Proceedings in Mathematics & Statistics, 64: 25-29. Link

Boncristiani H.F., Evans J.D., Chen Y., Pettis J., Murphy C., Lopez D.L., Simone-Finstroem M., Strand M., Tarpy D.R., Rueppell O. In-vitro infection of pupae with Israeli Acute Paralysis Virus suggests variation for susceptibility and disturbance of transcriptional homeostasis in honey bees (Apis mellifera). PLoS ONE, 8(9): e73429. Link


Rueppell O., Meier S., Deutsch R. Multiple mating but not recombination causes quantitative increase in offspring genetic diversity for varying genetic architectures. PLoS ONE, 7(10): e47220. Link

Page R.E., Fondrk M.K., Rueppell O. Complex pleiotropy characterizes the pollen hoarding syndrome in honey bees (Apis mellifera L.). Behavioral Ecology and Sociobiology, 66: 1459-1466. Link

Page R.E., Rueppell O., Amdam G.V. Genetics of reproduction and regulation of honey bee (Apis mellifera L.) social behavior. Annual Review of Genetics, 46: 97-119. Link

Dixon L.R., McQuage M.R., Lonon E.J., Buehler D., Seck O., Rueppell O. Pleiotropy of segregating genetic variants that affect honey bee worker life expectancy. Experimental Gerontology, 47: 631-637. Link

Munday M., Rinderer T.E., Rueppell O. Worker ovary size and activation of Russian honey bees (Apis mellifera L.). Journal of Apicultural Research, 51: 147-149. Link

Shorter J.R., Rueppell O.  (2012) A review on self-destructive defense behaviors in social insects. Insectes Sociaux, 59: 1-10. Link


Rueppell O., Hayes A.M., Warrit N., Smith D.R. Population structure of Apis cerana in Thailand reflects biogeography and current gene flow rather than Varroa mite association. Insectes Sociaux, 58: 445-452. Link

Rueppell O., Phaincharoen M., Kuster R.D., Tingek S. Cross-species correlation between queen mating numbers and worker ovary sizes suggests kin conflict may influence ovary size evolution in honeybees. Naturwissenschaften, 98: 795-799. Link

Graham, A.M., Munday, M.D., Kaftanoglu, O., Page R.E., Amdam G.V., Rueppell, O. Support for the reproductive ground plan hypothesis of social evolution and major QTL for ovary traits of Africanized worker honey bees (Apis mellifera L.). BMC Evolutionary Biology,11: 95. Link

Abbot, P., Abe, J., Alcock, J., et al. (Alphabetical List of 136 Authors including Rueppell, O.) Inclusive fitness theory and eusociality. Nature, 471: E1-E4. Link

Wilhelm M.E., Chhetri M., Rychtar J., Rueppell O. A game theoretical analysis of the mating sign behavior in the honey bee. Bulletin of Mathematical Biology, 73: 626-638. Link

Rueppell O., Metheny J.D., Linksvayer T.A., Fondrk M.K., Page R.E., Amdam G.V. Genetic architecture of ovary size and asymmetry in European honeybee workers. Heredity, 106: 894-903. Link

Willard L.E., Hayes A.M., Wallrichs M.A., Rueppell O. Food manipulation in honey bees induces physiological responses at the individual and colony level. Apidologie, 42:508-518. Link

Martinson, V., Danforth, B. N., Minckley, R., Rueppell, O., Tingek, S., Moran, N. A simple and distinctive microbiota exclusively associated with honey bees and bumble bees. Molecular Ecology, 20: 619-628. Link


Rueppell O., Hayworth M.K., Ross N.P. (2010) Altruistic self-removal of health-compromised honey bee workers from their hive. Journal of Evolutionary Biology, 23: 1538-1546. Link

Meznar E.R., Gadau J., Koeniger N., Rueppell O. Comparative linkage mapping suggests a high recombination rate in all honey bees. Journal of Heredity, 101: S118-S126. Link

Kalcounis-Rueppell M.C., Petric R., Briggs J. R., Carney C., Marshall M.M., Willse J.T., Rueppell O., Ribble D.O., Crossland J.P. Differences in ultrasonic vocalizations between wild and laboratory California mice (Peromyscus californicus). PLoS ONE, 5 (4): e9705. Link


Linksvayer T.A., Rueppell O., Siegel A., Kaftanoglu O., Page R.E., Amdam G.V.A. The genetic basis of transgressive ovary size in honey bee workers. Genetics, 183: 693-707. Link

Gove R.P., Hayworth M. K., Chhetri M., Rueppell O. Division of labour and social insect colony performance in relation to task and mating number under two alternative response threshold models. Insectes Sociaux, 56: 319-331. Link

Rueppell O., Kaftanoglu O., Page R.E. Honey bee (Apis mellifera) workers live longer in small than in large colonies. Experimental Gerontology, 44: 447-452. Link

Hayworth M. K., Johnson N.G., Wilhelm M.E., Gove R.P., Metheny J.D., Rueppell O. Added weights lead to reduced flight behavior and mating success in polyandrous honey bee queens (Apis mellifera). Ethology, 115: 698-706. Link

Rueppell O. Characterization of quantitative trait loci for the age of first foraging in honey bee workers. Behavior Genetics, 39: 541-553. Link

Amdam G.V., Rueppell O., Fondrk M.K., Page R.E., Nelson C.M. The nurse’s load: early-life exposure to brood-rearing affects behavior and lifespan in honey bees (Apis mellifera). Experimental Gerontology, 44: 467-471. Link

Viljakainen L., Evans J.D., Hasselmann M., Rueppell O., Tingek S., Pamilo P. Rapid evolution of immune proteins in social insects. Molecular Biology and Evolution, 26: 1791-1801. Link

Wang Y., Amdam G.V., Rueppell O., Wallrichs M.A., Fondrk M.K., Kaftanoglu O., Page R.E. PDK1 and HR46 gene homologs tie social behavior to ovary signals. PLoS ONE, 4(4): e4899. Link

Chhetri M., Johnson N., Rueppell O., Rychtar J. Revisiting the variance-based selection model of diploid drone production for multiple mating in honey bees. Journal of Interdisciplinary Mathematics, 12(2): 141-160. Link


Rueppell O., Hunggims E., Tingek S. Association between larger ovaries and pollen foraging in queenless Apis cerana workers supports the reproductive ground – plan hypothesis of social evolution. Journal of Insect Behavior, 21: 317-321. Link

Ward K.N., Coleman J., Clittin K., Fahrbach S., Rueppell O. Age, caste, and behavior determine the replicative activity of intestinal stem cells in honeybees (Apis mellifera L.). Experimental Gerontology, 43: 530-537. Link

Rueppell O., Linford R., Gardner P., Coleman J., Fine K. Aging and demographic plasticity in response to experimental age structures in honeybees (Apis mellifera L). Behavioral Ecology and Sociobiology, 62: 1621-1631. Link

Rueppell O., Johnson N., Rychtar J. Variance-based selection may explain general mating patterns in social insects. Biology Letters, 4: 270-273. Link


Rueppell O., Bachelier C., Fondrk M.K., Page R.E. Regulation of life history determines lifespan of worker honey bees (Apis mellifera L). Experimental Gerontology, 42: 1020-1032. Link

Rueppell O., Christine S., Mulcrone C., Groves L. Aging without functional senescence in honey bee workers. Current Biology, 17: R274-R275. Link

Hunt G., Amdam G.V., Schlipalius D., Emory C., Sardesai N., Williams C., Rueppell O., Guzmán-Novoa E., Arechavaleta-Velasco M., Chandra S., Fondrk K.M., Beye M., Page R.E. Behavioral genomics of honeybee foraging and nest defense. The potential of the honey bee for positional cloning: High recombination rates lead to targeted genome scans for foraging and defensive-behavior genes. Naturwissenschaften, 94: 247-267. Link


Beye M., Gattermeier I., Hasselmann M., Gempe T., Schioett M., Baines J., Schlipalius D., Mougel F., Emore C., Rueppell O., Sirviö A., Guzmán-Novoa E., Hunt G., Solignac M., Page R.E. Exceptionally high levels of recombination across the honey bee genome. Genome Research, 16: 1339-1344. Link

Rueppell O., Chandra S., Pankiw T., Fondrk M.K., Beye M., Hunt G., Page R.E. The genetic architecture of sucrose responsiveness in the honey bee (Apis mellifera L.). Genetics, 172: 243-251. Link

Sirviö A., Gadau J., Rueppell O., Lamatsch D., Boomsma J.J., Pamilo P., Page R.E. High recombination frequency creates genotypic diversity in colonies of the leaf-cutting ant Acromyrmex echinatior. Journal of Evolutionary Biology, 15: 1475-1485. Link

Rueppell O., Page R.E., Fondrk M.K. Male maturation response to selection of the pollen-hoarding syndrome in honey bees (Apis mellifera L.). Animal Behaviour, 71: 227-234. Link


Rüppell O., Kirkman R. Extraordinary starvation resistance in Temnothorax rugatulus (Hymenoptera, Formicidae) colonies: Demography and adaptive behavior. Insectes Sociaux, 52: 282-290. Link

Rueppell O., Fondrk M.K., Page R.E. Biodemographic analysis of male honey bee mortality. Aging Cell, 4: 13-19. Link


Rüppell O., Pankiw T., Page R.E. Pleiotropy, epistasis and new QTL: the genetic architecture of honey bee foraging behavior. Journal of Heredity, 95: 481-491. Link

Rueppell O., Pankiw T., Nielsen D.I., Fondrk M.K., Beye M., Page R.E. The genetic architecture of the behavioral ontogeny of foraging in honey bee workers. Genetics, 167: 1767-1779. Link