Nick Ader

Nick Ader

Assistant Professor

321B Eberhart Building


Cell Biology, Cellular Compartmentalization, Membrane Dynamics, Cellular Microscopy, Organelle Dynamics


Ph.D., University of Cambridge


Membranes are the bags that hold cells together. Within eukaryotic cells (like yours and mine), sub-compartments are formed by organelle membranes to allow specialized structures. The Ader Lab seeks to uncover how cells build and maintain these compartments. Projects in our lab investigate how cells maintain their existing compartments during dynamic cellular processes (such as nuclear envelope remodeling during mitosis) as well as how cells build compartments from scratch (such as plasma membrane biogenesis in fungal meiosis). We approach these investigations using a variety of techniques, such as live-cell fluorescence microscopy and correlative light and electron microscopy (CLEM) of the model yeast, S. Pombe. 

Recent Publications

Mannino, P.J., Perun, A.,† Surovstev, I.V., Ader, N.R., Shao, L., Melia, T.J., King, M.C., & Lusk, C.P. A quantitative ultrastructural timeline of nuclear autophagy reveals a role for dynamin-like protein 1 at the nuclear envelope. bioRxiv (2024) †Undergraduate researcher; pre-print

Ader, N.R., Chen, L.,† Surovstsev, I.V., Chadwick, W.L., Rodriguez, E.C., King, M.C., & Lusk, C.P. An ESCRT grommet cooperates with a diffusion barrier to maintain nuclear integrity. Nat Cell Biol (2023) †Undergraduate researcher; peer-reviewed research

Chandra, S.,* Mannino, P.J.,* Thaller., D.J.,* Ader, N.R., King, M.C., Melia, T.J., & Lusk, C.P. Atg39 selectively captures inner nuclear membrane into lumenal vesicles for delivery to the autophagosome. J Cell Biol (2021). *Co-first author; peer-reviewed research

Thaller, D.J., Tong, D., Marklew, C.J., Ader, N.R., Mannino, P.J., Borah, S., King, M.C., & Lusk, C.P. Direct binding of ESCRT protein Chm7 to phosphatidic acid-rich membranes at nuclear envelope herniations. J Cell Biol (2021) Peer-reviewed research

Lusk, C.P. & Ader, N.R. CHMPions of repair: emerging perspectives on sensing and repairing the nuclear envelope barrier. Curr Opin Cell Bio (2020) Review

Ader, N.R., Hoffmann, P.C., Ganeva, I., Borgeaud, A., Wang, C., Youle, R.J., & Kukulski, W. Molecular and topological reorganizations in mitochondrial architecture interplay during Bax- mediated steps of apoptosis. eLife (2019) Peer-reviewed research

Ader, N.R. & Kukulski, W. triCLEM: Combining high-precision, room temperature CLEM with cryo- fluorescence microscopy to identify very rare events. Methods Cell Biol (2017) Editorially reviewed method

Ader, N.R. Seeking an in vivo neuronal context for the PINK1/Parkin pathway. J Neurosci (2016) Commentary

Kemmerer, Z.A.*, Ader, N.R.*, Mulroy, S.S. & Eggler, A.L. Comparison of human Nrf2 antibodies: A tale of two proteins. Toxicol Lett (2015) *Co-first author; peer-reviewed research

Biscans, A., Bos, M., Martin, A.R., Ader, N.R., Szakiel, G., Vassear, J.J., Dupouy, C., & Depart, F. Direct synthesis of partially modified 2′-O-pivaloyloxymethyl RNAs by a base-labile protecting group strategy and their potential for prodrug-based gene-silencing applications. ChemBioChem (2014) Peer-reviewed research


Cell Biology (BIO 355)

Cell Biology and Genetics Lab (BIO 375)

Genetics (BIO 392)

Advanced Genetics (BIO 495)