Joseph Santin

Joseph Santin

Assistant Professor

(336) 334-4991
377 Sullivan Building


Cellular neuroscience, comparative neurobiology, electrophysiology


Ph.D., Wright State University


What we do: We answer both applied and basic questions about how the nervous system helps animals survive in their environments. On the applied side, we study animals that evolved ways to avoid damage to the nervous system. We focus most of our efforts on challenges to the nervous system that tend to be big problems in many human diseases. These include inactivity of neuromuscular systems (think, “if you don’t use it you lose it”) and impaired oxygen transport (think, brain damage in stroke and cardiac arrest). By learning from animals that already “know” how to get around these problems, we up our chances of finding new solutions. On the basic side, we use this approach to make new discoveries about how the nervous system helps animals adapt to their environments. To do this, we take fundamental concepts of cellular neurobiology that were developed outside of real-life contexts (e.g., cell culture, artificial lab settings, etc.) and test how they work in situations where animals may need them to survive. This allows us to put together new ideas about how these processes are important for behavior and why they may have evolved.

How we do it: We use both non-traditional (currently, frogs) and conventional animal models (rats and mice) in the lab. For a variety of reasons, we tend to focus on the neural systems that regulate breathing. On the technical side, we use an integrative approach that spans from whole animal behavior down to the molecular biology of single neurons. We use a range of tools that include patch clamp electrophysiology to study electrical properties of cells, single-cell quantitative PCR to study gene expression in individual neurons, in vivo measurements of behavior (measurements of breathing and EMG to record muscle activity), extracellular recording to assess circuit activity, and fluorescence imaging microscopy.

Research Opportunities: Graduate Students– We are looking for enthusiastic, creative, motivated graduate students (MS and PhD). A background in neurophysiology and general physiology is a plus. A background in animal surgery is a bigger plus. A background in patch clamp electrophysiology is an even bigger plus. If you are interested in joining us, please send an email to with your C.V. and two references. Please be sure to check out some of the papers listed below and give a description of why you’re interested in joining the lab. Undergraduates– We are also interested in helping undergraduate students gain hands-on research experience in neuroscience. Hands-on research goes beyond learning laboratory techniques and passively doing experiments. If you are interested in asking questions about the topics described here, designing a project, and learning research tools to answer the question, please contact Dr. Santin.

Recent Publications:

Santin, J.M. Motor inactivity in hibernating frogs: Linking plasticity that stabilizes neuronal function to behavior in the natural environment. Developmental Neurobiology. 2019, in press doi:

Santin, J.M., Schulz, D.J. Membrane voltage is a direct feedback signal that influences correlated ion channel expression in neurons. Current Biology. 2019, 29; 1683–1688, doi: 10.1016/j.cub.2019.04.008

Vajello, M., Santin, J.M., Hartzler, L.K. Noradrenergic modulation determines respiratory network activity during temperature changes in the in vitro brainstem of bullfrogs. Respiratory Physiology and Neurobiology. 2018, 258; 25-31. doi:10.1016/j.resp.2018.10.002

Kempf, E.A., Rollins, K.S., Hopkins, T.D., Butenas, A.L., Santin, J.M., Smith, J.R., Copp, S.W. Chronic femoral artery ligation exaggerates the pressor and sympathetic nerve responses during dynamic skeletal muscle stretch in decerebrate rats. American Journal of Physiology- Heart and Circulatory Physiology. 2018. 314; H246-H254 doi: 10.1152/ajpheart.00498.2017

Santin, J.M. How important is CO2 chemoreflex for the control of breathing? Environmental and evolutionary considerations.  Comparative Biochemistry and Physiology, Part A: Molecular and Integrative Physiology. 2018; 215, 6-19. doi: 10.1016/j.cbpa.2017.09.015

Santin, J.M., Vajello, M., Hartzler, L.K.  Synaptic up-scaling preserves motor circuit output after chronic, natural inactivity. eLife. 2017; 6:e30005. doi: 10.7554/eLife.30005  

Santin, J.M., Hartzler L.K. Activation of respiratory muscles does not occur during cold-submergence in bullfrogs, Lithobates catesbeianus. The Journal of Experimental Biology. 2017; 220, 1181-1186. doi: 10.1242/jeb.153544

Santin, J.M., Hartzler, L.K. Environmentally-induced return to juvenile-like chemosensitivity in the respiratory control system of adult bullfrog, Lithobates catesbeianusThe Journal of Physiology-London. 2016; 594; 6349-6367. doi: 10.1113/JP272777

Santin, J.M., Wang, T., Dukkipati, S.S., Hartzler, L.K. Commentary: The spinal cord has an intrinsic system for the control of pH. Frontiers in Physiology.  2016; 7:513. doi: 10.3389/fphys.2016.00513

Santin, J.M., Hartzler, L.K. Control of breathing following overwintering conditions in the American bullfrog, Lithobates catesbeianus. The Journal of Experimental Biology.  2016; 219; 2003-2016. doi: 10.1242/jeb.136259

Santin, J.M., Hartzler, L.K. Reassessment of chemical control of breathing in bullfrogs, Lithobates catesbeianus, using measurements of pulmonary ventilation. Respiratory Physiology & Neurobiology. 2016; 224, 80-89. doi: 10.1016/j.resp.2015.09.013

Santin, J.M., Hartzler, L.K. Activation state of the hyperpolarization-activated current (Ih) modulates temperature-sensitivity of firing in locus coeruleus neurons from bullfrogs. American Journal of Physiology- Regulatory, Integrative, & Comparative. 2015; 308, R1045-1061. doi: 10.1152/ajpregu.00036.2015

Imber, A.N., Santin, J.M., Graham, C.D., Putnam, R.W. A HCO3-dependent mechanism involving soluble adenylyl cyclase for the activation of Ca2+ currents in locus coeruleus neurons. Biochimica et Biophysica Acta. 2014; 1842; 2569-2578. doi : 10.1016/j.bbadis.2014.07.027

Santin, J.M., Watters, K.C., Putnam, R.W., Hartzler, L.K. Temperature influences neuronal activity and CO2/pH sensitivity of locus coeruleus neurons in the bullfrog, Lithobates catesbeianus. American Journal of Physiology- Regulatory, Integrative, & Comparative. 2013; 305; R1451-R1464. doi: 10.1152/ajpregu.00348.2013

 Santin, J.M., Hartzler, L.K. Respiratory signaling of locus coeruleus neurons during hypercapnic acidosis in the bullfrog, Lithobates catesbeianus. Respiratory Physiology & Neurobiology 2013;185, 553-561. doi: 10.1016/j.resp.2012.11.002

For a full list of publications see my Google Scholar profile: